Constant expansion of the capabilities of global navigation systems provided for consumers allows geodetic methods of positioning to develop intensively. One of the promising methods of positioning in the field of geodesy and navigation is the Moving Base method. The purpose of the article is to obtain experimentally and estimate the relative positioning accuracy of that method. Within the framework of the research, an experiment was conducted to determine the accuracy of relative positioning in the Moving Base mode. To estimate the accuracy during the experiment, the results of processing GNSS measurements performed on a special measuring stand based on a metrological accurate rigid basis as well as two multi-frequency GNSS smart antennas as main equipment for the experimental measurements were used. The measurements were carried out, both in the fixed position, and during the movement of the basis. The article describes in detail the experimental program survey routes, as well as gives an explanation of selection. The results of the experiment were processed, as well as for visualization of all the obtained values. All of them are presented in the form of detailed graphs. In the course of the work, based on the results of the experiment, conclusions about several factors’ influence on the quality of positioning in the Moving base method were drawn. According to the results of the conducted research, the obtained accuracy of determining the length of the base under different working conditions were also summed up.